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Automating DNA Library Preparation of Small Genomes using Illumina ® DNA Preparation Kit on the Opentrons OT-2

The next-generation sequencing (NGS) workflow includes preparing DNA libraries using commercially available and cost-effective DNA preparation kits. Optimizing this workflow by using an automated OT-2 protocol helps to improve the efficiency for DNA library preparation. Read More

Rapid High Quality Next Generation Sequencing Library Preparation With Swift 2S Turbo DNA Library Kits© On The Opentrons OT-2

The Swift 2S™ Turbo DNA Library Kit was automated with the Opentrons OT-2 for fast and robust preparation of high quality libraries for next generation sequencing. Read More

Affordable COVID-19 Testing Automation With The Opentrons OT-2

Using the Opentron’s OT-2 and platform, we demonstrate a highly sensitive, affordable, and hands-off pipeline for PCR-based SARS-CoV-2 testing. Read More

Automated, Rapid & Reproducible Measurement Of Immunoglobulin G Using Opentrons OT-2, Valita®TITER and Valita®TITER Plus

Biologic drugs are the largest and fastest growing segment of the Pharmaceutical industry, with sales of €500bn and an annual growth of 8%.1 Their ability to target diseases with high specificity results in improved safety and effectiveness. However, this increases the cost of manufacturing significantly over their predecessors. Read More

Robust and high-throughput SARS-CoV-2 viral RNA detection, research, and sequencing using RNAdvance Viral and the OT-2 platform

Here we demonstrate an automated SARS-CoV-2 RNA extraction workflow using a Beckman Coulter Life Sciences RNAdvance Viral kit (C63510) on an Opentrons OT-2 robot; an open-source robotic liquid handling platform. Read More

Automated library preparation of small genomes using DNA preparation kits (Illumina DNA Prep Kit, KAPA HyperPrep kit, and NEBNext Ultra II Kit)

Three library prep kits – Illumina® DNA Prep Kit, KAPA® HyperPrep kit, and NEBNext® Ultra™ II Kit – were automated with the Opentrons OT-2 for fast and robust preparation of high quality libraries for next generation sequencing. Read More

Automating Sensitive Polymerase Chain Reaction Protocols Using the Opentrons Thermocycler on the Opentrons OT-2 Platform

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note, we demonstrate that the Opentrons Thermocycler and the OT-2 generate the expected base-pair length for PCR amplicons when compared to a popular off-deck third-party thermocycler. Read More

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