Three library prep kits – Illumina® DNA Prep Kit, KAPA® HyperPrep kit, and NEBNext® Ultra™ II Kit – were automated with the Opentrons OT-2 for fast and robust preparation of high quality libraries for next generation sequencing. Read More

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note, we demonstrate that the Opentrons Thermocycler and the OT-2 generate the expected base-pair length for PCR amplicons when compared to a popular off-deck third-party thermocycler. Read More

An automated protocol was developed to perform dual flow chromatography using Biotage® PhyTip® columns on the Opentrons OT-2 liquid handler. Read More

An automated protocol was developed to perform magnetic bead-based immobilized metal affinity chromatography (IMAC) on the Opentrons OT-2 liquidhandler. Read More

Automated protocols developed to perform magnetic bead-based immunoprecipitation (IP) to process up to 96 samples with minimized hands-on-time were tested on the robotic liquid handler Opentrons OT-2. Read More

DNA clean-up and size selection steps for next-generation sequencing (NGS) workfowsrequire precise and accurate pipetting, which can be labor intensive for large scaleexperiments. Omega Bio-tek’s Mag-Bind® TotalPure NGS Kit provides a fully automatable,highly fexible solution for both DNA and RNA clean-up and size selection. The OmegaBio-tek Mag-Bind® TotalPure NGS Kit… Read More