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Automating Sensitive Polymerase Chain Reaction Protocols Using the Opentrons Thermocycler on the Opentrons OT-2 Platform

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note, we demonstrate that the Opentrons Thermocycler and the OT-2 generate the expected base-pair length for PCR amplicons when compared to a popular off-deck third-party thermocycler. Read More

Automated Protein Purification with Biotage Dual Flow Chromatography PhyTip Columns on the OT-2

An automated protocol was developed to perform dual flow chromatography using Biotage® PhyTip® columns on the Opentrons OT-2 liquid handler. Read More

Automated Ni-NTA bead-based protein purification on the OT-2 liquid handler

An automated protocol was developed to perform magnetic bead-based immobilized metal affinity chromatography (IMAC) on the Opentrons OT-2 liquidhandler. Read More

Immunoprecipitation using Thermo Fisher Dynabeads on the OT-2

Automated protocols developed to perform magnetic bead-based immunoprecipitation (IP) to process up to 96 samples with minimized hands-on-time were tested on the robotic liquid handler Opentrons OT-2. Read More

Fully Automated NGS Clean-up and Size Selection with Omega Bio-Tek Mag-Bind® TOTALPURE NGS on the OT-2

DNA clean-up and size selection steps for next-generation sequencing (NGS) workfowsrequire precise and accurate pipetting, which can be labor intensive for large scaleexperiments. Omega Bio-tek’s Mag-Bind® TotalPure NGS Kit provides a fully automatable,highly fexible solution for both DNA and RNA clean-up and size selection. The OmegaBio-tek Mag-Bind® TotalPure NGS Kit… Read More