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Automated NGS Quantification, Normalization, and Pooling Using the OT-2

The Opentrons® OT-2 effectively performs qPCR quantification of NGS libraries, maintaining high precision while reducing hands-on time. Two methods of sample normalization were tested: individually for each sample or pooled with enzymatic normalization. Read More

Volatile Liquid Handling with Opentrons OT-2

Volatile liquids, liquids that evaporate readily at room temperature, are common reagents in various applications. Read More

Viscous Liquid Handling Automation using Opentrons OT-2

Molecular assays require precise pipetting reagents and samples with a diverse set of properties — including viscous ones that can clog tips, distribute unevenly and complicate reproducibility… Read More

Automation of bead-based ELISA forSARS-CoV-2 neutralizing antibody on the Opentrons OT-2 with an on-deck Heater-Shaker Module

Upon recognition of viral components, cell-mediated immune responses trigger secretion of neutralizing antibodies against viral antigens, for example nucleocapsid (N) protein and spike (S) protein present on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Read More

Executing Serial Dilutions On The OT-2

Serial dilutions are both independent experiments and building blocks of more complex experimental pipelines. Applications can be as simple as generating a standard curve or more complex phenotype characterization across cell culture concentrations. In any application, precise pipetting is required to generate reliable results. Read More

High performance automated nucleic acid extraction with the OT-2 platform

Modern molecular biology, driven by ever progressing technology, continues to deliver impressive and exciting new advancements — larger studies, more powerful therapeutics, and wholly new research questions. Read More

Sequence Ready Library Preparation Fully Automated For Microbial And Human DNA Template

The Swift 2S Turbo DNA Library Kit was fully automated using the Opentrons OT-2 for quick, hands-off, highquality libraries for Illumina Next-Generation Sequencing. Read More

Microbiome Nucleic Acid Extraction with ZymoBIOMICS Kit on OT-2

Microbiomics is a burgeoning field with implications for a wide range of topics in human health. Modern sequencing techniques can deliver rich and novel data but are constrained by DNA extraction methods that are difficult, time consuming, and, especially in microbiomics samples, prone to bias. Read More

Automation of three ELISA protocols on the Opentrons OT-2 with an on-deck Heater-Shaker Module

Enzyme immunoassays (EIAs) or enzyme-linked immunosorbent assays (ELISAs) are widely used analytic tools for detection of a target molecule (e.g., an antigen) in liquid samples for diagnostic or research purposes. Read More

Opentrons COVID-19 Testing System One Sheeter

The Opentrons COVID-19 Testing System is a flexible, high-throughput surveillance testing system using OT-2 hardware & validated withZymo reagents to process up to 2,400 samples per day. Read More